| Group | Club/Team Name | Team ID | Head Coach |
| D21 | Beach United FC - 01 Beach United FC Flash |
03022-001AG0U-1295 | Thomas Harrison |
| D23 | Charleston Soccer Club LLC CSC 15 Girls Navy North |
03034-001AG0U-0027 | Benjamin Justice |
| D24 | FC Charleston FC Charleston 2015/16 Girls 2 |
03046-001JG0U-0009 | Russell Jackson |
| D25 | Florence Soccer Association - 01 FSA 2015 Girls |
03043-001JG0U-0012 | Chelsea Parker |
| D26 | James Island Youth SC JIYSC 2015 Girls Red Moreno |
03001-001AG0U-0002 | Mary Moreno |
| D27 | Kickoff Soccer Academy - 01 KOSA 2015/2016 Girls Black |
03033-001AG0U-0019 | Lester Shelley |
| D28 | Charleston Soccer Club LLC CSC 15 Girls Navy South II |
03034-001AG0U-0029 | John Dreger |
| D31 | Charleston Soccer Club LLC CSC 16 Girls Navy North |
03034-001AG9U-0038 | Megan McCue-Jones |
| D32 | James Island Youth SC JIYSC 2016 Girls Red Smith |
03001-001AG9U-0010 | Gregg Smith |
| D33 | GSC-Bluffton United 2015 Girls | 03012-001RG0U-2046 | Erika Vargas |
| D34 | FC Charleston FC Charleston 2015/16 Girls 1 |
03046-001JG0U-0008 | Nick Rivera |
| D35 | Mt. Pleasant FC - 01 MPFC U10 Jaguars |
03007-001RG0U-0011 | Gordon Shuford |
| D36 | Daniel Island SA DISA U10G Jr. Academy |
03030-001AG0U-0006 | Alexander Riviere |
| D37 | Charleston United SC - 01 Charleston United '15/'16 Girls Blue |
03002-001RG0U-0126 | Jurgen Wagner |
| D38 | Cainhoy Athletic Soccer Club CASC 15 Blue |
03006-001AG0U-2405 | Michael Johnson |